Journal: Cell & Bioscience

Article Title: Scorpion venom peptide SPVII promotes irradiated cells proliferation and increases the expression of the IL-3 receptor

doi: 10.1186/2045-3701-3-28

Figure Lengend Snippet: SVPII and SVPIII enhanced proliferation of irradiated M-NFS-60 cells (applied alone or with IL-3). M-NFS-60 cells were irradiated for 5 min (5 Gy total dose) and then treated with PBS (control), 3 mg/L SVPII for 48 h (II), 3 mg/ml SVPIII for 48 h (III), 10 μg/L IL-3 for 48 h (IL-3), or the indicated combinations (II + IL-3, III + IL-3). * P < 0.01 vs. control; # P < 0.05 vs. II or vs. III).

Article Snippet: Recombinant human macrophage colony stimulating factor (rhM-CSF) and recombinant mouse (rm) IL-3 were purchased from PeproTech Co. AlamarBlue™ was purchased from AbD Serotec (Great Britain), and membrane protein isolation kits were from Bio-Rad.

Techniques: Irradiation

Journal: Cell & Bioscience

Article Title: Scorpion venom peptide SPVII promotes irradiated cells proliferation and increases the expression of the IL-3 receptor

doi: 10.1186/2045-3701-3-28

Figure Lengend Snippet: Effect of SVPII on CFU count on 11th and 14th days in vitro (magnification: ×100). BM-MNCs (1 × 10 6 cells/mL) of BALB/C mouse were treated by: A: PBS (Control), B: cytokines (10 μg/L IL-3 and 62 μg/L rhM-CSF), C: 1 mg/L SVPII, D: 3 mg/L SVPII, E: 1 mg/L SVPII + cytokines, F: 3 mg/L SVPII + cytokines. Treated BM-MNCs were added into methyl cellulose half-solid medium and CFU was count on the 11th and 14th days.

Article Snippet: Recombinant human macrophage colony stimulating factor (rhM-CSF) and recombinant mouse (rm) IL-3 were purchased from PeproTech Co. AlamarBlue™ was purchased from AbD Serotec (Great Britain), and membrane protein isolation kits were from Bio-Rad.

Techniques: In Vitro

Journal: Cell & Bioscience

Article Title: Scorpion venom peptide SPVII promotes irradiated cells proliferation and increases the expression of the IL-3 receptor

doi: 10.1186/2045-3701-3-28

Figure Lengend Snippet: Influence of SVPII on IL-3 receptor expression in M-NFS-60 cells (% IL-3R + cells). Control: cells treated with solvent (PBS); II: cells treated with 3 mg/L SVP II; IL-3: cells treated with 10 μg/L IL-3; II + IL-3: cells treated with 3 mg/L SVP II and 10 μg/L IL-3 simultaneously. * P < 0.05 vs. control group.

Article Snippet: Recombinant human macrophage colony stimulating factor (rhM-CSF) and recombinant mouse (rm) IL-3 were purchased from PeproTech Co. AlamarBlue™ was purchased from AbD Serotec (Great Britain), and membrane protein isolation kits were from Bio-Rad.

Techniques: Expressing

Journal: Cell & Bioscience

Article Title: Scorpion venom peptide SPVII promotes irradiated cells proliferation and increases the expression of the IL-3 receptor

doi: 10.1186/2045-3701-3-28

Figure Lengend Snippet: Immunofluorescence images demonstrating the influence of SVPII on IL-3 R expression by M-NFS-60 cells (×200). A. Control: cells treated with solvent (PBS); B. IL-3: cells treated with 10 μg/L IL-3; C. SVPII: cells treated with 3 mg/L SVP II; D. SVPII+IL-3: cells treated with 3 mg/L SVP II and 10 μg/L IL-3 simultaneously.

Article Snippet: Recombinant human macrophage colony stimulating factor (rhM-CSF) and recombinant mouse (rm) IL-3 were purchased from PeproTech Co. AlamarBlue™ was purchased from AbD Serotec (Great Britain), and membrane protein isolation kits were from Bio-Rad.

Techniques: Immunofluorescence, Expressing

Journal: Cell & Bioscience

Article Title: Scorpion venom peptide SPVII promotes irradiated cells proliferation and increases the expression of the IL-3 receptor

doi: 10.1186/2045-3701-3-28

Figure Lengend Snippet: Influence of SVPII on IL-3R expression by M-NFS-60 cells. A. Normal culture condition: 10%FCM + rhM-CSF 62 μg/L); B. Low concentration of M-CSF: 10%FCM + rhM-CSF 15.5 μg/L; 1. Control: cells treated with solvent(PBS); 2. IL-3: cells treated with 10 μg/L IL-3; 3.SVPII: cells treated with 3 mg/L SVP II; 4. SVPII + IL-3: cells treated with 3 mg/L SVP II and 10 μg/L IL-3 simultaneously.

Article Snippet: Recombinant human macrophage colony stimulating factor (rhM-CSF) and recombinant mouse (rm) IL-3 were purchased from PeproTech Co. AlamarBlue™ was purchased from AbD Serotec (Great Britain), and membrane protein isolation kits were from Bio-Rad.

Techniques: Expressing, Concentration Assay

Journal: Cell & Bioscience

Article Title: Scorpion venom peptide SPVII promotes irradiated cells proliferation and increases the expression of the IL-3 receptor

doi: 10.1186/2045-3701-3-28

Figure Lengend Snippet: Influence of SVPII on IL-3 R expression of M-NFS-60 cells after irradiation (% IL-3R + cells). After irradiated, the cells were treated with solvent(control) or 3 mg/L SVP II(II) or 10 μg/L IL-3(IL-3) or with 3 mg/L SVP II and 10 μg/L IL-3 simultaneously(II + IL-3). * P < 0.05 vs. control.

Article Snippet: Recombinant human macrophage colony stimulating factor (rhM-CSF) and recombinant mouse (rm) IL-3 were purchased from PeproTech Co. AlamarBlue™ was purchased from AbD Serotec (Great Britain), and membrane protein isolation kits were from Bio-Rad.

Techniques: Expressing, Irradiation

Journal: Stem Cell Research & Therapy

Article Title: Anti-inflammatory and vasculogenic conditioning of peripheral blood mononuclear cells reinforces their therapeutic potential for radiation-injured salivary glands

doi: 10.1186/s13287-019-1414-7

Figure Lengend Snippet: Contents in semisolid culture for EPC-CFA

Article Snippet: rm IL-3 , Peprotech, #213-13 , 20 ng/mL.

Techniques: Concentration Assay